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Herpesviruses and bacteriophages Virology research

Virology research plays an important role in developing vaccines, antiviral drugs and diagnostic tests for viral infections. Our Virology research team led by Darby Oldenburg, PhD, focuses on finding answers for those whose lives are changed by herpesviruses and bacteriophages. 

Herpesviruses research

Like you, we want to understand how herpesviruses infections lead to autoimmune disease and cancer. We’re trying to find answers through our mutagenesis program, which provides collaborators with virus strains to better understand the role of each gene in the herpesvirus infection cycle and its latency.

Herpesvirus infections caused by Epstein Barr Virus and Kaposi’s Sarcoma Herpesvirus can lead to cancer, while infections caused by HHV-6 and EBV may play an important role in the development of autoimmune diseases.  

Our goal is to help identify the molecular pathways that lead to tumors and/or autoimmune disorders so that we can find ways to prevent cancer and change people’s lives. 

Bacteriophages research

Drug-resistant bacterial infections are rising, and the development of antibiotics to treat them is lagging. We’re joining other medical researchers pursuing Phage Therapy to learn whether bacteriophages have the power to treat bacterial infections.

We're curating a collection of bacteriophages against numerous bacterial pathogens and focusing our research on antibiotic-resistant Staphylococcus infections that can cause limb loss or worse. 

Two virology research scientists in lab.

Support our Virology research

We focus on developing basic molecular research tools that can help us understand the impact of herpesvirus infections. We're also building tools to create a future in which Phage Therapy can fight resistant life-threatening bacterial infections. It's because of people like you that Gundersen Medical Foundation can support our work to find answers and make a difference.

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  • 10

    collaborators around the country using new research tools we created

  • 50

    bacteriophages that infect Staphylococcus curated

  • 10

    years of mentorship between our researchers and undergrad microbiology students

Virology researchers

Research scientist darby oldenburg in lab.
Senior research scientist

Darby Oldenburg, PhD

(608) 775-4866

Email Dr. Oldenburg
Gundersen research scientist using equipment in lab.jpg
Research technician

Justin Radomski

Email Justin

Publications

The amplitude of gammaherpesvirus lytic replication dictates adaptive immune activation: Potential implications for KSHV LANA in immune evasion
Published on: 2026-02-12

Adaptive immune responses to primary Kaposi sarcoma-associated herpesvirus (KSHV) infection are poorly defined. To develop better small-animal models for understanding KSHV pathogenesis and immunity, we previously generated a chimeric virus in which the KSHV latency-associated nuclear antigen (kLANA), a conserved multifunctional protein critical for viral latency, was exchanged for the LANA homolog in murine gammaherpesvirus 68 (MHV68). Despite comparable levels of latent infection between WT...

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Inhibition of type I interferon signaling is a conserved function of gamma-herpesvirus-encoded microRNAs
Published on: 2025-12-31

Herpesviruses encode multiple factors that disarm innate immune signaling to evade host anti-viral responses. Several viral microRNAs expressed by Epstein-Barr virus (EBV) and Kaposi's sarcoma-associated herpesvirus (KSHV) disrupt the induction of type I interferons (IFN) and/or the subsequent signaling events activated through type I IFN receptors. Here, we aimed to determine whether non-human primate (NHP) γ-herpesviruses (rhesus lymphocryptovirus [rLCV], rhesus rhadinovirus [RRV], and...

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Intrinsic p53 activation restricts gammaherpesvirus driven germinal center B cell expansion during latency establishment
Published on: 2025-01-22

Gammaherpesviruses are DNA tumor viruses that establish lifelong latent infections in lymphocytes. For viruses such as Epstein-Barr virus and murine gammaherpesvirus 68, this is accomplished through a viral gene-expression program that promotes cellular proliferation and differentiation, especially of germinal center B cells. Intrinsic host mechanisms that control virus-driven cellular expansion are incompletely defined. Using a small-animal model of gammaherpesvirus pathogenesis, we demonstrate...

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Vaccination with a Replication-Dead Murine Gammaherpesvirus Lacking Viral Pathogenesis Genes Inhibits WT Virus Infection
Published on: 2025-01-13

Gammaherpesviruses are oncogenic pathogens that establish lifelong infections. There are no FDA-approved vaccines against Epstein-Barr virus or Kaposi sarcoma herpesvirus. Murine gammaherpesvirus-68 (MHV68) infection of mice provides a system for investigating of gammaherpesvirus pathogenesis and testing vaccine strategies. Prime-boost vaccination with a replication-dead virus (RDV) that does not express the essential replication and transactivator protein (RTA) encoded by ORF50 (RDV-50.stop)...

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Vaccination with a Replication-Dead Murine Gammaherpesvirus Lacking Viral Pathogenesis Genes Inhibits WT Virus Infection
Published on: 2025-01-08

Gammaherpesviruses are oncogenic pathogens that establish lifelong infections. There are no FDA-approved vaccines against Epstein-Barr virus or Kaposi sarcoma herpesvirus. Murine gammaherpesvirus-68 (MHV68) infection of mice provides a system for investigating gammaherpesvirus pathogenesis and testing vaccine strategies. Prime-boost vaccination with a replication-dead virus (RDV) that does not express the essential replication and transactivator protein (RTA) encoded by ORF50 (RDV-50.stop)...

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La Crosse, WI 54601

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